Top/fop flash实验

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August undefined, 2024

http://www.bioon.com.cn/reagent/Show_product.asp?id=3497789 Web9. feb 2024 · RNA-seq analysis, TOP/FOP flash reporter assay, western blot and transwell assay were performed to investigate the underlying mechanism for the function of RHBDD1 on Wnt signaling pathway. Bioinformatics analysis was conducted to investigate epithelial-mesenchymal transition (EMT) and stemness in HCT-116 cells. ...

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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96420 WebMillipore luciferase top flash Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … russian cat ear cleaning

miRNA-101通过靶向CDK8调控结肠癌细胞侵袭和Wnt/β-catenin通 …

WebThe results of TOP/FOP flash experiments showed that the activity of luciferase was decreased by the transfection of MEG3 overexpression vector compared with the control group, while the activity of luciferase was increased after transfecting with MEG3 interference vector (*p0.05). MEG3, maternally expressed gene 3. Web5. júl 2024 · TOP/FOP-Flash报告基因实验表明:敲低或过表达CDX2能显著增加或降低Wnt信号传导活性。 Western Blot实验结果表明:在CDX 2基因敲低或过表达的结肠癌细胞中,wnt … Web1．实验第一天，消化并接种细胞（根据具体实验选择合适的细胞）于35mm细胞培养皿，置于5％CO2、饱和湿度的37℃培养箱内培养过夜。 2．等细胞密度达到70％时用Luciferase报告基因质粒、LacZ的表达质粒及其它质粒（根据具体实验确定）共转染细胞（根据具体实验选择转染方法，如磷酸钙沉淀法、PEI、lipofectamine2000等均可）。 3．转染24－36h … schedule analysis best practices

Supplementary Information Materials and Methods - Journal of …

WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 … WebThe best answers are voted up and rise to the top Home Public; Questions; Tags Users Unanswered Teams. Stack Overflow for Teams – Start collaborating and sharing organizational knowledge. Create a free Team Why Teams? Teams. Create free Team … schedule an advising appointment ohio stateWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... schedule a naat test

"WebResearchers who want to detect active Wnt-ßcatenin signaling in cells have various tools available. To measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse and ... " - Top/fop flash实验

Top/fop flash实验

Web具体实验流程：注：该操作流程通常用来检测转染了萤火虫荧光素酶基因的真核细胞中荧光素酶表达的活性，不适用于对细菌荧光素酶进行检测。 1．实验第一天，消化并接种细 … Web13. apr 2024 · Acer Aspire 5 与 i3-1115G4, UHD Graphics Xe G4, 15.6”, Full HD (1920 x 1080), IPS, 256GB SSD, 8GB DDR4, Windows 11 Pro, Rock eDigital 32GB USB3.0 Flash Drive 的规格、测试和价格。

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Web1. sep 2015 · Top和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP …

Web3、荧光素酶报告基因检测只需要成功将含有目标DNA序列插入到报告载体中，即可进行后续实验，Western blotting则需要购买商品化的一抗，一般价高量少，且抗体的杂交条件需要摸索；若自制的一抗，抗体效价需要验证，实验周期较长。 WebSupplementary Information Materials and Methods TOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites …

WebJ-STAGE Home Web如果实验室没有的话可以用别的仪器取代吗？或者说，用别的实验思路去取代这个实验一下可以吗？我的实验是想做某一抑癌基因对wnt经典通路的影响，可不可以使抑癌基因在某 …

Web功能实验表明，miR-504过表达抑制了GBM细胞的恶性行为，例如迁移，侵袭，EMT和干活性。此外，miR-504通过直接抑制FZD7表达而成为Wnt-β-catenin途径的负调节剂，而FZD7过表达逆转了由miR-504引起的EMT抑制。

Web12. máj 2024 · Common targets: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Cyclophilin B (PPIB), and Lamin (LMNA) Negative Controls Negative controls are designed to have no known computationally derived target in the cells being transfected and are commonly called a scrambled control. schedule analysis methodWeb具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和 … schedule analysis method formWeb具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧 … schedule analysis method self employedWeb25. máj 2024 · TOPFlash载体基本信息订购信息质粒图谱载体描述 The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate … schedule analysis reportWeb信号通路报告载体TOPFlash FOPFlash SuperTopFlash是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂，产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的信号通路报告载体TOPFlash FOPFlash SuperTopFlash试剂销售服务商之一，在海淀区等地方销售信号通路报告载体TOPFlash FOPFlash SuperTopFlash试剂 ... schedule analysis softwareWeb目的. 研究VPS26在高脂环境中对大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSC）成骨、成脂分化作用的机制，探讨VPS26对高脂大鼠种植体骨结合和裸鼠异位成骨的影响。 schedule analyst job descriptionWebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 … schedule amvets pick up san diego