Web1 Adenine 18 2 p-Aminobenzoic acid 8 3 Leucine 380 4 Alanine 76 5 Arginine 76 6 Asparagine 76 7 Aspartic acid 76 8 Cysteine 76 9 Glutamic acid 76 10 Glutamine 76 11 Glycine 76 12 Histidine 76 13 myo-Inositol 76 14 Isoleucine 76 15 Lysine 76 16 Methionine 76 17 Phenylalanine 76 18 Proline 76 19 Serine 76 20 Threonine 76 WebApr 18, 2014 · The four vectors were linearized with AflII (NEB) and 3 μg of each plasmid were transformed into electrocompetent cells of P. pastoris PichiaPink™ Strain 4. The …
Comparative Analysis of Mesophilic YqfB-Type Amidohydrolases
WebOct 15, 2015 · The culture was then spread onto PAD (pichia adenine dropout) agar plate and incubated for 7–10 days at 24–30 °C. 2.6.1. Conditions used Better transformation efficiency was found at the OD 600 of 1 at the primary inoculum and OD600 of 1.3–1.5 in the secondary inoculum. WebSep 1, 2024 · After electroporation in a Gene Pulser Xcell System (BIORAD), YPDS media was added to electroporated cells and incubated for 2 h at 30 °C. The culture was then spread onto PAD (pichia adenine dropout) agar plate plus 500 µg/ml G418 and incubated at 30 °C until white distinct colonies are formed. details flight ek751 weight tocket
Comparative Analysis of Mesophilic YqfB-Type Amidohydrolases
WebThe PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Yeast Expression System. The PichiaPink™ Media Kit contains media needed to grow Pichia pastoris strains from frozen stocks to cultures ready for transformation and selection. WebAug 1, 2016 · The selectable marker of pPinkα-HC plasmid is ade2 gene (adenine synthase); therefore, selection of transformants was carried out on PAD (Pichia Adenine Dropout) agar plates. Presence of SXA gene in the yeast genome was verified by colony PCR using SXA specific primers. WebMar 8, 2013 · P. pastoris were incubated with YPDS media (YPD with 1 M sorbitol) in the Gene Pulser Cuvettes at 28°C for 2 hr without shaking, spread onto PAD (Pichia Adenine Dropout) agar selection plates, and then incubated at 28°C … detail section in revit