WebThe cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. … WebpCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。 该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry和绿色荧光蛋白GFP双重标签的LC3B的融合蛋白，呈现明亮的 微信在线咨询 扫码加微信 快速订购 联系我们 选择语 …

pCMV-mCherry-GFP-LC3B(pCMV-mCherry-GFP-LC3B质 …

Web1 uur geleden · To assess the stability of NΔCit1, we performed a cycloheximide chase experiment and found that NΔCit1 was degraded with a half-life of ~45 min but was stabilized by the addition of the proteasome inhibitor MG132 ( Fig. 2E and fig. S3B). WebFor this purpose we cloned mCherry ( BBa_J06504) into the purification and expression vector pTXB1 from NEB and at the same time added six histidines to the C-terminus of … chicken and bacon

Light-induced protein degradation in human-derived cells

WebmCherry is een lid van de mFruits-familie van monomere rode fluorescerende eiwitten (mRFP's). Als een RFP was mCherry afgeleid van DsRed van Discosoma … WebmCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with … WebDisclosed is a patch or bandage for tissue regeneration and/or repair. The bandage comprises i) one or more proteins from the Wnt family, or an agonist of the Wnt signalling pathway; and ii) a scaffold, wherein the one or more Wnt proteins or Wnt agonist is immobilised on the scaffold, and wherein the scaffold is formed from a functionalised … chicken and baby marrow recipe