Bodipy-labeled palmitate
WebApr 14, 2024 · This finding was confirmed by using alkyne-labeled palmitic acid (alkyne-palmitate), which can be detected upon click reaction with an azide-coupled fluorescent reporter (Gaebler et al., 2016) ... BODIPY-labeled FAs have been previously used to study lipid trafficking and can be esterified into neutral lipids and stored in LDs (Herms et al., ... WebLabeling by 13 C palmitate can be found in multiple metabolites, confirming efficient labeling ( Figure 7 A). Interestingly, A20 myel-KO mice show significantly more palmitate …
Bodipy-labeled palmitate
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WebThe labeled fat can be observed as lipids, fatty acids (FA) and TG, in plasma, chylomicrons (CM), and very low-density lipoprotein (VLDL) particles. Dietary TG absorption reaches …
WebThe QBT Fatty Acid Uptake Assay uses a BODIPY®-dodecanoic acid fluorescent fatty acid analog coupled with our proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids and is a known substrate for fatty acid transporters. WebBODIPY-Palmitate is a fluorescently tagged form of palmitic acid (Item No. 10006627) that displays excitation/emission maxima of 488/508 nm, respectively. 1,2 It has been used to …
WebMay 19, 2024 · A mask following the shape of the lymphatic vessel (LYVE1+ staining) was applied to isolate the lipid droplets (LDs) signal (BODIPY 493/503) coming from the LYVE1+ lymphatic vessel (dashed... WebWe used boron dipyrromethene difluoride (BODIPY)-labeled palmitate to show that leptin (60 nM) caused a time-dependent (0-60 min) increase in fatty acid uptake in L6 myoblasts. Quantitative analysis using 3H-palmitate showed that pre-incubation with insulin (100 nM, 24 h) prevented stimulation of fatty acid uptake by leptin.
WebJan 21, 2024 · We show that stimulation with a CpG oligodeoxynucleotide, a Toll-like receptor 9 agonist, evokes changes in the central carbon metabolism of macrophages that enable antitumor activity, including...
WebJan 5, 2024 · Specifically, we developed a quantitative optical approach to spatially and longitudinally map the kinetics of long-chain fatty acid uptake in in vivo murine models of … team chat exportierenWebAug 6, 2024 · To measure FFA uptake in skeletal muscle cells, we adopted a BODIPY-palmitate protocol [ 26] for cultured skeletal myoblasts. L6 GLUT4myc WT cells were grown on 96-well plates overnight at a density of 1.6 × 10 4 cells/well and, following serum-starvation, treated with insulin or ionomycin as described above. team chat everyoneWebJul 8, 2024 · Our group has previously shown that it is possible to quantify fatty acid uptake and glucose uptake in multiple tissues, simultaneously, using a commercially available boron-dipyrromethene... teamchat gamingWebApr 21, 2011 · A bodipy probe was developed for site-specific labeling of tagged proteins inside live cells which displays a large spectral change upon covalent coupling to the … team chat filesWebJun 3, 2024 · Medical Definition of Palmitate. Palmitate: An antioxidant and a vitamin A compound that is added to low-fat and fat-free milk to replace the vitamin content lost … southwest general hospital newsWebApr 28, 2006 · In this study, we show that BODIPY FL C16 binds to purified liver and intestinal fatty acid-binding proteins with high affinity at a site similar to that for the … southwest general hospital pediatricsWebFeb 11, 2024 · (D–F) BODIPY (D and E) and oil red O (F) staining in Hilpdaflox/flox and HilpdaΔMΦ BMDMs lipid loaded with oleate:palmitate or BSA (Cntl) for 24 h. Data are shown from or are representative of at least three independent experiments. southwest general hospital visiting hours